Journal: Oncotarget

Article Title: Ino80 promotes cervical cancer tumorigenesis by activating Nanog expression

doi: 10.18632/oncotarget.12667

Figure Lengend Snippet: ChIP-qPCR analysis showing Ino80 binding levels in the Oct4 and Nanog gene TSS and upstream sites in HeLa cells ( A ) Enrichment fold changes are normalized to IgG binding and is represented as mean±SEM ( n = 3). *** p < 0.001. qRT-PCR ( B ) and western blotting ( C ) analysis of Nanog in control and Ino80 knockdown HeLa cells. Western blot analysis used GAPDH as a loading control. ChIP-qPCR analysis showing Ino80 binding levels in the Nanog TSS in control and Ino80 knockdown HeLa cells ( D ) Data in B and D are represented relative to controls as means ± SEM ( n = 3). *** p < 0.001.

Article Snippet: Primary antibodies targeting NANOG (Abcam), Ino80 (proteintech, Chicago, IL), OCT4 (Santa Cruz Biotechnology, Santa Cruz, CA), p53 (Santa Cruz Biotechnology), BAX (Abcam) and GAPDH (Santa Cruz Biotechnology) were incubated with the proteins overnight at 4°C, followed by incubation with the appropriate HRP (horseradish peroxidase)-conjugated secondary antibodies.

Techniques: ChIP-qPCR, Binding Assay, Quantitative RT-PCR, Western Blot, Control, Knockdown

Journal: Oncotarget

Article Title: Ino80 promotes cervical cancer tumorigenesis by activating Nanog expression

doi: 10.18632/oncotarget.12667

Figure Lengend Snippet: Cells are divided into three groups: HeLa cell (control), Ino80 knockdown HeLa cell (shIno80 A) and Nanog overexpression in Ino80 knockdown HeLa cell (shIno80 A + Nanog). qRT-PCR ( A ) and western blotting ( B ) analysis of Nanog in the shIno80 A and shIno80 A + Nanog groups. qRT-PCR data are displayed relative to the shIno80 A group as mean ± SEM ( n = 3). *** p < 0.001. Western blot analysis used GAPDH as a loading control. Control, shIno80 A and shIno80 A + Nanog HeLa cell growth curves constructed from CCK-8 assay results ( C ). Data are represented as means ± SEM ( n = 3). * p < 0.05, ** p < 0.01. Colony formation in control, shIno80 A and shIno80 A + Nanog HeLa cells ( D ). Colonies were stained with crystal violet. Ki67 expression in control, shIno80 A and shIno80 A + Nanog HeLa cells as determined by IF staining ( E ) Nuclei were counterstained with DAPI. Bars = 40 μm.

Article Snippet: Primary antibodies targeting NANOG (Abcam), Ino80 (proteintech, Chicago, IL), OCT4 (Santa Cruz Biotechnology, Santa Cruz, CA), p53 (Santa Cruz Biotechnology), BAX (Abcam) and GAPDH (Santa Cruz Biotechnology) were incubated with the proteins overnight at 4°C, followed by incubation with the appropriate HRP (horseradish peroxidase)-conjugated secondary antibodies.

Techniques: Control, Knockdown, Over Expression, Quantitative RT-PCR, Western Blot, Construct, CCK-8 Assay, Staining, Expressing

Journal: Molecular cell

Article Title: Methyl-Metabolite Depletion Elicits Adaptive Responses to Support Heterochromatin Stability and Epigenetic Persistence

doi: 10.1016/j.molcel.2020.03.004

Figure Lengend Snippet: Data and Software Availability

Article Snippet: TruSeq DNA Nano , Illumina , Cat#20015964.

Techniques: Software, Recombinant, Sequencing, Modification, Extraction, Reverse Transcription, Multiplex sample analysis, Plasmid Preparation, SYBR Green Assay, Staining, cDNA Synthesis, DNA Purification, Purification, Control, Targeted Proteomics

Journal: Molecular cell

Article Title: Methyl-Metabolite Depletion Elicits Adaptive Responses to Support Heterochromatin Stability and Epigenetic Persistence

doi: 10.1016/j.molcel.2020.03.004

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: TruSeq DNA Nano , Illumina , Cat#20015964.

Techniques: Recombinant, Sequencing, Modification, Extraction, Reverse Transcription, Multiplex sample analysis, Plasmid Preparation, SYBR Green Assay, Staining, cDNA Synthesis, DNA Purification, Purification, Control, Software, Targeted Proteomics